HOXC4 Antibody (OTI2D5)

Novus Biologicals | Catalog # NBP3-44812

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D5
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Product Specifications

Immunogen

Full length human recombinant protein of human HOXC4 (NP_705897) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for HOXC4 Antibody (OTI2D5)

HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Western Blot;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Western Blot;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY HOXC4 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC4 (1:2000). Positive lysates
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemistry;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
HOXC4 Antibody (OTI2D5) Western Blot;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Western Blot;HOXC4 Antibody (OTI2D5)[NBP3-44812]-

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-HOXC4 monoclonal antibody (1:500).

Applications for HOXC4 Antibody (OTI2D5)

Application
Recommended Usage

Immunohistochemistry

1:2000

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOXC4

HOXC4 belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene, HOXC4, is one of several homeobox HOXC genes located in a cluster on chromosome 12. Three genes, HOXC5, HOXC4 and HOXC6, share a 5' non-coding exon. Transcripts may include the shared exon spliced to the gene-specific exons, or they may include only the gene-specific exons. Two alternatively spliced variants that encode the same protein have been described for HOXC4. Transcript variant one includes the shared exon, and transcript variant two includes only gene-specific exons.

Alternate Names

homeo box 3E, homeo box C4, homeobox C4, Homeobox protein CP19, Homeobox protein Hox-3E, homeobox protein Hox-C4, HOX3, HOX3Ecp19

Gene Symbol

HOXC4

Additional HOXC4 Products

Product Documents for HOXC4 Antibody (OTI2D5)

Certificate of Analysis

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Product Specific Notices for HOXC4 Antibody (OTI2D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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