HPV16 E1+E4 Antibody (TVG 403) - BSA Free

Novus Biologicals | Catalog # NB100-2767

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TVG 403

Format

BSA Free
View all HPV16 E1+E4 Primary Antibodies »

Product Specifications

Immunogen

Antigen for hybridoma production was expressed as a beta galactosidase fusion protein using an expression system and was consequently cleaved to release the E1/E4 polypeptide.

Reactivity Notes

Human papilloma virus

Localization

Nuclear

Specificity

The epitope recognized by this antibody survived brief fixation in 5% formaldehyde, but not prolonged fixation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for HPV16 E1+E4 Antibody (TVG 403) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPV16 E1+E4

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Alternate Names

ATP dependent helicase E1, E1, E4, HPV 16 ATP dependent helicase E1, HPV 16 E1, HPV 16 E4, HPV 16 replication protein E1, HPV16 ATP dependent helicase E1, HPV16 E1, HPV16 E4, HPV16 replication protein E1, Human papilloma virus type 16, Human papilloma virus type 16 ATP dependent helicase E1, Human papilloma virus type 16 E1, Human papilloma virus type 16 E4, Human papilloma virus type 16 replication protein E1, Human papillomavirus type 16, Human papillomavirus type 16 ATP dependent helicase E1, Human papillomavirus type 16 E1, Human papillomavirus type 16 E4, Human papillomavirus type 16 replication protein E1, Replication protein, Replication protein E1

Additional HPV16 E1+E4 Products

Product Documents for HPV16 E1+E4 Antibody (TVG 403) - BSA Free

Certificate of Analysis

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Product Specific Notices for HPV16 E1+E4 Antibody (TVG 403) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HPV16 E1+E4 Antibody (TVG 403) - BSA Free

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  • Q: I was wondering if the sequence recognized by the antibody TVG 402 (NB100-2766) corresponds to the E1 sequence or to the E4 sequence? As in, would this antibody recognize either E4 or E1 if they are not found as a E1^E4 fusion protein?

    A: According to our product page the immunogen says : Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.HEre is the original paper describing the characterization of this clone (TVG 402): https://www.sciencedirect.com/science/article/pii/004268229290327L?via%3Dihub

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