HPV18 E7 Antibody (GT881) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13610
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT881
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length HPV18 E7 recombinant protein.
Reactivity Notes
Human papillomavirus type 18
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for HPV18 E7 Antibody (GT881) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: HPV18 E7 Antibody (GT881) [NBP3-13610]
Immunocytochemistry/Immunofluorescence: HPV18 E7 Antibody (GT881) [NBP3-13610] - HPV18 E7 antibody [GT881] detects HPV18 E7 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPV18 E7 stained by HPV18 E7 antibody [GT881] (NBP3-13610) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.ELISA: HPV18 E7 Antibody (GT881) [NBP3-13610] -
ELISA: HPV18 E7 Antibody (GT881) [NBP3-13610] - Sandwich ELISA detection of recombinant HPV18 E7 protein, His tag using antibodies as below.Capture: HPV18 E7 antibody [HL2522] (5 ug/mL)
Detection: HPV18 E7 antibody [GT881] (NBP3-13610) (1 ug/mL)
ELISA: HPV18 E7 Antibody (GT881) [NBP3-13610] -
ELISA: HPV18 E7 Antibody (GT881) [NBP3-13610] - Sandwich ELISA detection of recombinant HPV18 E7 protein, His tag using antibodies as below.Capture: HPV18 E7 antibody [GT881] (NBP3-13610) (5 ug/mL)
Detection: HPV18 E7 antibody [HL2522] (1 ug/mL)
Western Blot: HPV18 E7 Antibody (GT881) [NBP3-13610] -
Various whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [GT881] (NBP3-13610) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Western Blot: HPV18 E7 Antibody (GT881) [NBP3-13610] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [GT881] (NBP3-13610) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Western Blot: HPV18 E7 Antibody (GT881) [NBP3-13610] -
Various whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [GT881] (NBP3-13610) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for HPV18 E7 Antibody (GT881) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Lateral Flow Assay
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HPV18 E7
Alternate Names
E7 protein
Additional HPV18 E7 Products
Product Documents for HPV18 E7 Antibody (GT881) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HPV18 E7 Antibody (GT881) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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