HSD11B2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37898

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 11 beta-HSD2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GDMPYPCLGAYGTSKAAVALLMDTFSCELLPWGVKVSIIQPGCFKTESVRNVGQWEKRKQLLLANLPQELLQAYGKDYIEHLHGQFLHSLR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HSD11B2 Antibody - BSA Free (NBP2-37898) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSD11B2 Antibody - BSA Free

Western Blot: HSD11B2 Antibody [NBP2-37898]

Western Blot: HSD11B2 Antibody [NBP2-37898]

Western Blot: HSD11B2 Antibody [NBP2-37898] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Kidney tissue
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human rectum.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining in human kidney and liver tissues using anti-HSD11B2 antibody. Corresponding HSD11B2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human kidney, liver, lymph node and rectum using Anti-HSD11B2 antibody NBP2-37898 (A) shows similar protein distribution across tissues to independent antibody NBP2-37954 (B).
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898]

Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human lymph node.

Applications for HSD11B2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 11 beta-HSD2

HSD11B2 catalyzes the conversion of cortisol to the inactive metabolite cortisone. The protein modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.

Long Name

11 beta Hydroxysteroid Dehydrogenase 2

Alternate Names

11 betaHSD2, HSD11B2

Gene Symbol

HSD11B2

UniProt

P80365

Additional 11 beta-HSD2 Products

Product Documents for HSD11B2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSD11B2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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