HSF2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86330

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSF2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLETTKNNVVQPVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNPASSVEQASTTASSEVLSSVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HSF2 Antibody - BSA Free (NBP1-86330) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSF2 Antibody - BSA Free

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330] - Staining of human testis shows weak to moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330]

Immunohistochemistry-Paraffin: HSF2 Antibody [NBP1-86330] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
HSF2 Antibody - BSA Free Western Blot: HSF2 Antibody - BSA Free [NBP1-86330]

Western Blot: HSF2 Antibody - BSA Free [NBP1-86330]

Analysis in human cell lines HEK293 and HeLa using Anti-HSF2 antibody. Corresponding HSF2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
HSF2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: HSF2 Antibody - BSA Free [NBP1-86330]

Chromatin Immunoprecipitation-exo-Seq: HSF2 Antibody - BSA Free [NBP1-86330]

ChIP-Exo-Seq composite graph for Anti-HSF2 (NBP1-86330) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for HSF2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSF2

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Gene Symbol

HSF2

Additional HSF2 Products

Product Documents for HSF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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