HSP10/EPF Antibody - BSA Free

Novus Biologicals | Catalog # NB110-11473

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Rabbit, Sheep, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP10/EPF Primary Antibodies »

Product Specifications

Immunogen

Human Cpn10 peptide AA 91-101

Localization

Mitochondrial

Specificity

Rabbit Polyclonal to Cpn10

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP10/EPF Antibody - BSA Free

Western Blot: HSP10/EPF Antibody [NB110-11473]

Western Blot: HSP10/EPF Antibody [NB110-11473]

Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry: HSP10/EPF Antibody [NB110-11473]

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473]

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - Immunohistochemistry analysis using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..
Western Blot: HSP10/EPF Antibody [NB110-11473]

Western Blot: HSP10/EPF Antibody [NB110-11473]

Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry: HSP10/EPF Antibody [NB110-11473]

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473]

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - analysis using Rabbit Anti-Cpn10 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..

Applications for HSP10/EPF Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP10/EPF

Homologs of E. coli Cpn10 make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Cpn10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Cpn10 as a potential contributor to an immunological response.

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Entrez Gene IDs

3336 (Human); 15528 (Mouse); 25462 (Rat)

Gene Symbol

HSPE1

UniProt

P61604

Additional HSP10/EPF Products

Product Documents for HSP10/EPF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HSP10/EPF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Customer Reviews for HSP10/EPF Antibody - BSA Free

There are currently no reviews for this product. Be the first to review HSP10/EPF Antibody - BSA Free and earn rewards!

Have you used HSP10/EPF Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HSP10/EPF Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am wondering if the HSP10 with catalog number NB110-11473 is a primary (capture antibody) suitable for human sample? Also do you have pairs antibody for HSP60, HSP70?

    A:

    With regards to your question, NB110-11473 is a rabbit polyclonal antibody which also cross reacts with human. We do have the HSP60 and HSP70 antibodies for ELISA as well. If you are interested in ordering these antibodies, please find more detailed information in the following links. H00003329-AP21 and H00003308-PW1.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies