HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13617

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT357

Format

Azide and BSA Free
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser82 of human HSP27. The exact sequence is proprietary.

Modification

p Ser82

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser82] Antibody (GT357) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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