HSP40/DNAJB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03458

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP40/DNAJB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human HSP40/DNAJB1 (NP_006136.1). MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP40/DNAJB1 Antibody - BSA Free

HSP40/DNAJB1 Antibody - BSA Free

Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] -

Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Western blot analysis of lysates from 293T cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Knockout Validated: HSP40/DNAJB1 Antibody - BSA Free [NBP3-03458]

Western Blot: HSP40/DNAJB1 Antibody - BSA Free [NBP3-03458]

Western Blot: HSP40/DNAJB1 Antibody [NBP3-03458] - Analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using HSP40/DNAJB1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
HSP40/DNAJB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] -

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of U2OS cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
HSP40/DNAJB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] -

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of C6 cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
HSP40/DNAJB1 Antibody - BSA Free

Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] -

Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Western blot analysis of various lysates using [KO Validated] HSP40/DNAJB1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
HSP40/DNAJB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] -

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of HeLa cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for HSP40/DNAJB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP40/DNAJB1

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Long Name

Heat Shock 40 kDa Protein

Alternate Names

DNAJB1, Hdj1, HSPF1

Gene Symbol

DNAJB1

Additional HSP40/DNAJB1 Products

Product Documents for HSP40/DNAJB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP40/DNAJB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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