HSP60 Antibody (LK1)

Novus Biologicals | Catalog # NBP2-32973

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Hamster, Monkey, Rabbit, Sheep, Xenopus

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LK1
View all HSP60 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HSP60 protein (Uniprot: P10809)

Epitope

aa 383-447

Reactivity Notes

Does not react with Bacteria, helminths or spinach.

Localization

Mitochondria in cytoplasm

Marker

Mitochondrial Marker

Specificity

Recognizes a 60kDa protein, identified as the heat shock protein 60 (HSP60). Its epitope is localized between aa 383-447 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34670)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for HSP60 Antibody (LK1)

Western Blot: HSP60 Antibody (LK1) [NBP2-32973]

Western Blot: HSP60 Antibody (LK1) [NBP2-32973]

Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973]

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973] - Immunofluorescent staining of PFA-fixed MCF-7 cells. followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).
Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP60 MAb (LK1)
Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973]

Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973]

Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using HSP60 antibody (LK1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (LK1).
Simple Western: HSP60 Antibody (LK1) [NBP2-32973]

Simple Western: HSP60 Antibody (LK1) [NBP2-32973]

Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: HSP60 Antibody (LK1) [NBP2-32973]

Simple Western: HSP60 Antibody (LK1) [NBP2-32973]

Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.

Applications for HSP60 Antibody (LK1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa and HepG2 lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 61 kDa

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HSP60

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, arthrosclerosis and chlamydial disease.

Long Name

Heat Shock Protein 60

Alternate Names

cpn60, GROEL, HSPD1, SPG13

Gene Symbol

HSPD1

UniProt

P10809 (Mouse)

Additional HSP60 Products

Product Documents for HSP60 Antibody (LK1)

Certificate of Analysis

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Product Specific Notices for HSP60 Antibody (LK1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for HSP60 Antibody (LK1)

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Protocols

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