HSP70/HSPA1A Antibody (OTI3G9)

Novus Biologicals | Catalog # NBP2-02190

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G9
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP70/HSPA1A Antibody (OTI3G9)

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.
Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)
Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.

Applications for HSP70/HSPA1A Antibody (OTI3G9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (OTI3G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail