HSP90 beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55509

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP90 beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DEDEVAAEEPNAAVPDEIPPLEGDEDASR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Knockout (KO) Validated Rabbit HSP90 beta Antibody - BSA Free (NBP2-55509) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSP90 beta Antibody - BSA Free

Knockout Validated: HSP90 beta Antibody [NBP2-55509]

Western Blot: HSP90 beta Antibody [NBP2-55509]

Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HSP90 beta Antibody [NBP2-55509]

Western Blot: HSP90 beta Antibody [NBP2-55509]

Western Blot: HSP90 beta Antibody [NBP2-55509] - Analysis using Anti-HSP90AB1 antibody NBP2-55509 (A) shows similar pattern to independent antibody NBP2-68978 (B).
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody [NBP2-55509]

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody [NBP2-55509]

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Applications for HSP90 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP90 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail