HSPA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86185

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSPA2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24899640).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPA2 Antibody - BSA Free

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Analysis in human testis and pancreas tissues. Corresponding HSPA2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185]

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
HSPA2 Antibody - BSA Free Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Analysis in human testis tissue.
HSPA2 Antibody - BSA Free Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
HSPA2 Antibody - BSA Free Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Western Blot: HSPA2 Antibody - BSA Free [NBP1-86185]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for HSPA2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 24899640). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPA2

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediatethe folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participatein all these processes through their ability to recognize nonnative conformations of other proteins. They bindextended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membranetranslocation, or following stress-induced damage

Long Name

Heat Shock Protein A2

Alternate Names

HSP70-2

Gene Symbol

HSPA2

Additional HSPA2 Products

Product Documents for HSPA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSPA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for HSPA2 Antibody - BSA Free

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Protocols

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FAQs

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