HspA6 Antibody (OTI1H3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70959

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H3

Format

Azide and BSA Free
View all HSPA6/HSP70B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA6 (NP_002146) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

70.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HspA6 Antibody (OTI1H3) - Azide and BSA Free

Western Blot: HspA6 Antibody (OTI1H3)Azide and BSA Free [NBP2-70959]

Western Blot: HspA6 Antibody (OTI1H3)Azide and BSA Free [NBP2-70959]

Western Blot: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HspA6.
Immunocytochemistry/ Immunofluorescence: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959]

Immunocytochemistry/ Immunofluorescence: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959]

Immunocytochemistry/Immunofluorescence: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HspA6.
Flow Cytometry: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959]

Flow Cytometry: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959]

Flow Cytometry: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HspA6 antibody, and then analyzed by flow cytometry.
Western Blot: HspA6 Antibody (OTI1H3)Azide and BSA Free [NBP2-70959]

Western Blot: HspA6 Antibody (OTI1H3)Azide and BSA Free [NBP2-70959]

Western Blot: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HspA6 monoclonal antibody.

Applications for HspA6 Antibody (OTI1H3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSPA6/HSP70B

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Heat Shock Protein A6

Alternate Names

HSP70B

Gene Symbol

HSPA6

Additional HSPA6/HSP70B Products

Product Documents for HspA6 Antibody (OTI1H3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HspA6 Antibody (OTI1H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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