HSPA8/HSC71/Hsc70 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16882

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%), Rhesus Macaque (100%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSPA8/HSC71 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA8/HSC71/Hsc70. The exact sequence is proprietary.

Localization

Cytoplasm; Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody - BSA Free

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.
Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.
HSPA8/HSC71/Hsc70 Antibody - BSA Free

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] -

Hsc70 antibody detects Hsc70 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Hsc70 antibody (NBP2-16882) dilution: 1:5000
HSPA8/HSC71/Hsc70 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] -

HSC70 antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSC70 protein stained by HSC70 antibody (NBP2-16882) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for HSPA8/HSC71/Hsc70 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP2-16882 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

UniProt

P11142 (Human)

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Heat Shock Proteins (HSPs)

Customer Reviews for HSPA8/HSC71/Hsc70 Antibody - BSA Free (1)

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1 Customer Rating
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Customer Images

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  • HSPA8/HSC71/Hsc70 Antibody
    Name: Kevin Schauer
    Application: Western Blot
    Sample Tested: Adult small intestine
    Species: Opsanus beta
    Verified Customer | Posted 02/15/2017
    Gulf toadfish (Opsanus beta) intestinal protein extract was run on a 4-15% acrylamide gel. Blot was incubated overnight at 4C in antibody diluted 1:500.
    HSPA8/HSC71/Hsc70 Antibody - BSA Free NBP2-16882

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Protocols

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