HSPB8/HSP22 Antibody (OTI1E3)

Novus Biologicals | Catalog # NBP2-02576

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E3
View all HSPB8 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPB8/HSP22 Antibody (OTI1E3)

Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Immunocytochemistry/Immunofluorescence: Hsp22 Antibody (1E3) [NBP2-02576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.
Flow Cytometry: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Flow Cytometry: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Hsp22 antibody, and then analyzed by flow cytometry.
Western Blot: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Western Blot: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hsp22.
Flow Cytometry: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Flow Cytometry: HSPB8/HSP22 Antibody (OTI1E3) [NBP2-02576]

Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - Analysis of Jurkat cells, using anti-Hsp22 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for HSPB8/HSP22 Antibody (OTI1E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.88 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq].

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Entrez Gene IDs

26353 (Human)

Gene Symbol

HSPB8

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody (OTI1E3)

Certificate of Analysis

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Product Specific Notices for HSPB8/HSP22 Antibody (OTI1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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