HSPB8/HSP22 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30922

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSPB8 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSPB8/HSP22. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPB8/HSP22 Antibody - BSA Free

Western Blot: HSPB8/HSP22 Antibody [NBP1-30922]

Western Blot: HSPB8/HSP22 Antibody [NBP1-30922]

Western Blot: HSPB8/HSP22 Antibody [NBP1-30922] - Sample (30 ug whole cell lysate) A:293T whole cell lysateB:A431 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-30922]

Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-30922]

Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-30922] - Analysis of HeLa, using NBP1-30922 at 1:200 dilution.
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-30922]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-30922]

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-30922] - Human lung SCC, using HSP22 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for HSPB8/HSP22 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Gene Symbol

HSPB8

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HSPB8/HSP22 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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