HT 8-oxo-dG ELISA Kit II

R&D Systems | Catalog # 4380-192-K

For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.
R&D Systems
Discontinued Product
4380-192-K has been discontinued. View all 8-oxo-dG products.

Key Product Details

Features

Quantifies 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.

Key Benefits

  • Colorimetric, non-radioactive format
  • High throughput 96 strip wells
  • Dynamic range from 3.13 nM to 200 nM (0.89 ng/mL to 56.7 ng/mL)
  • Sensitivity at 2 nM (0.57 ng/mL) 8-OHdG

Species

Multi-Species

Product Summary for HT 8-oxo-dG ELISA Kit II

Product Specifications
For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.
Other Resources
4380-096-k_calculation_worksheet

Kit Contents for HT 8-oxo-dG ELISA Kit II

  • 8-OHdG Standard, 20 µM
  • 8-OHdG Monoclonal Antibody
  • Goat anti-Mouse IgG-HRP
  • DNase I (5 Units/µl)
  • Alkaline Phosphatase (1 Unit/µl)
  • Assay Dilutent
  • 100X Cations
  • Pre-coated 96-stripwell plate
  • TACS-Sapphire

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: 8-oxo-dG

The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

Long Name

8-Hydroxyguanine

Alternate Names

8-OHG, 8oxodG

Additional 8-oxo-dG Products

Product Documents for HT 8-oxo-dG ELISA Kit II

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Related Research Areas

Base Excision Repair

Citations for HT 8-oxo-dG ELISA Kit II

Customer Reviews for HT 8-oxo-dG ELISA Kit II (4)

4 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

Have you used HT 8-oxo-dG ELISA Kit II?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • HT 8-oxo-dG ELISA Kit II
    Name: Anonymous
    Verified Customer | Posted 09/06/2023
    Catalog No. 4380-096-P (Contains 1 plate only) LOT : P324064 Sample type being tested: Human Plasma The standard curve came out well. However, our plasma samples were diluted 1/10 suggested by the kit. Our OD values were out of range. If you have enough plates, I would suggest to perform a standardization run, to determine optimal dilution for your samples first.
    HT 8-oxo-dG ELISA Kit II 4380-192-K
  • HT 8-oxo-dG ELISA Kit II
    Name: Lindsie Morin
    Verified Customer | Posted 09/29/2020
    Very sensitive assay that needs vigorous vortexing, but overall does produce good results.
    HT 8-oxo-dG ELISA Kit II 4380-192-K
  • HT 8-oxo-dG ELISA II Kit, 2 Plates
    Name: Anonymous
    Verified Customer | Posted 12/17/2018
    HT 8-oxo-dG ELISA Kit II 4380-192-K
  • HT 8-oxo-dG ELISA II Kit, 2 Plates
    Name: Anonymous
    Verified Customer | Posted 09/18/2018
    HT 8-oxo-dG ELISA Kit II 4380-192-K

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Activity Assays