Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 496214
View all 5-HT2C Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human 5-HT2C
Accession # P28335

Specificity

Detects human 5-HT2C in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human 5‑HT2C APC‑conjugated Antibody

Detection of 5-HT2C antibody in HEK293 Human Cell Line Transfected with Human 5-HT2C and eGFP antibody by Flow Cytometry.

Detection of 5‑HT2C in HEK293 Human Cell Line Transfected with Human 5-HT2C and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human 5-HT2C and eGFP was stained with either (A) Mouse Anti-Human 5-HT2C APC-conjugated Monoclonal Antibody (Catalog # FAB5764A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human 5‑HT2C APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human 5-HT2C and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5-HT2C

5-HT2C is a 45 kDa 7TM receptor that belongs to the G-protein coupled receptor #1 family. It binds serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5-HT2C ligation regulates mood, appetite, and motivation. 5-HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5-HT2C can be generated by RNA editing. Human 5-HT2C shares 89% aa sequence identity with mouse and rat 5-HT2C.

Long Name

5-Hydroxytryptamine Receptor 2C

Alternate Names

5-HTR2C, 5HT2C, HTR2C, Serotonin Receptor 2C

Entrez Gene IDs

3358 (Human); 15560 (Mouse); 25187 (Rat)

Gene Symbol

HTR2C

UniProt

P28335

Additional 5-HT2C Products

Product Documents for Human 5‑HT2C APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 5‑HT2C APC‑conjugated Antibody

For research use only

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