Human 5-HT2C Antibody

Catalog #: MAB5764 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5764
MAB5764-SP

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Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 5-HT2C Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5HT2C in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 496214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human 5HT2C

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Immunohistochemistry

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5HT2C in Human Brain. 5HT2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT2C

5HT2C is a 45 kDa 7TM receptor for serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5HT2C ligation regulates mood, appetite, and motivation. 5HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5HT2C can be generated by RNA editing. Human 5HT2C shares 89% aa sequence identity with mouse and rat 5HT2C.

Long Name

5-Hydroxytryptamine Receptor 2C

Entrez Gene IDs

3358 (Human); 15560 (Mouse); 25187 (Rat)

Alternate Names

5-HT1C; 5-HT-1C; 5HT2C; 5-HT2C; 5-HT-2C; 5-HT2C5-hydroxytryptamine receptor 1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C; 5-hydroxytryptamine receptor 2C; HTR1C; HTR2C; serotonin 5-HT-2C receptor; Serotonin Receptor 2C