Human 5-HT2C Antibody

Catalog # Availability Size / Price Qty
MAB5764
MAB5764-SP
Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry.
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Human 5-HT2C Antibody Summary

Species Reactivity
Human
Specificity
Detects human 5HT2C in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 496214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human 5HT2C
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry. View Larger

Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

Immunohistochemistry 5HT2C in Human Brain. View Larger

5HT2C in Human Brain. 5HT2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT2C

5HT2C is a 45 kDa 7TM receptor for serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5HT2C ligation regulates mood, appetite, and motivation. 5HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5HT2C can be generated by RNA editing. Human 5HT2C shares 89% aa sequence identity with mouse and rat 5HT2C.

Long Name
5-Hydroxytryptamine Receptor 2C
Entrez Gene IDs
3358 (Human); 15560 (Mouse); 25187 (Rat)
Alternate Names
5-HT1C; 5-HT-1C; 5HT2C; 5-HT2C; 5-HT-2C; 5-HT2C5-hydroxytryptamine receptor 1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C; 5-hydroxytryptamine receptor 2C; HTR1C; HTR2C; serotonin 5-HT-2C receptor; Serotonin Receptor 2C

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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