Human A33 Antibody [Biotin]

R&D Systems | Catalog # BAF3080

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all A33 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Specificity

Detects human A33 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human A33 Antibody [Biotin]

Detection of A33 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon carcinoma cell line was stained with Goat Anti-Human A33 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3080, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Applications for Human A33 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HT‑29 human colon carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human A33

Reviewed Applications

Read 1 review rated 5 using BAF3080 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol

GPA33

UniProt

Q99795

Additional A33 Products

Product Documents for Human A33 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human A33 Antibody [Biotin]

For research use only

Customer Reviews for Human A33 Antibody [Biotin] (1)

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  • Human A33 Biotinylated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT-29 human colon carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/26/2021
    Human A33 Antibody [Biotin] BAF3080

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