Human A33 Biotinylated Antibody Summary
Accession # Q99795
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon carcinoma cell line was stained with Goat Anti-Human A33 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3080, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
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