Human A33 Antibody Summary
Accession # Q99795
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human A33 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of A33 in TH‑29 Human Cell Line by Flow Cytometry. TH‑29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human A33 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for A33 at approximately 64 kDa (as indicated) using 20 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2, and JAM-A, -B, and -C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD shares 80%, 74%, and 71% aa sequence identity with canine, bovine, and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
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