Human A33 Antibody

Catalog # Availability Size / Price Qty
AF3080
AF3080-SP
Detection of A33 in TH‑29 Human Cell Line by Flow Cytometry.
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Human A33 Antibody Summary

Species Reactivity
Human
Specificity
Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human A33
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of A33 in TH‑29 Human Cell Line by Flow Cytometry. View Larger

Detection of A33 in TH‑29 Human Cell Line by Flow Cytometry. TH‑29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry A33 in Human Colon. View Larger

A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2, and JAM-A, -B, and -C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD shares 80%, 74%, and 71% aa sequence identity with canine, bovine, and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name
Cell Surface A33 Antigen
Entrez Gene IDs
10223 (Human); 59290 (Mouse)
Alternate Names
A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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