Home / PTP1B / Human Active PTP1B DuoSet IC, 2 Plate

Human Active PTP1B DuoSet IC, 2 Plate

Catalog #: DYC1625-2
1 Citation Datasheet / COA / SDS

Discontinued Product

DYC1625-2 has been discontinued.
View all PTP1B products.
Product Details
Citations (1)
FAQs
Reviews
Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human Active PTP1B DuoSet IC, 2 Plate Summary

Assay Type
ELISA Development Kit
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) dephosphorylates tyrosine residues on several proteins including c-Src, beta-catenin, and the receptors for insulin and epidermal growth factor. PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play a role in regulating growth factor responsiveness.

Long Name:
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs:
5770 (Human); 19246 (Mouse)
Alternate Names:
EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1

Citation for Human Active PTP1B DuoSet IC, 2 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Recurrent somatic mutations of PTPN1 in primary mediastinal B cell lymphoma and Hodgkin lymphoma.
    Authors: Gunawardana J, Chan F, Telenius A, Woolcock B, Kridel R, Tan K, Ben-Neriah S, Mottok A, Lim R, Boyle M, Rogic S, Rimsza L, Guiter C, Leroy K, Gaulard P, Haioun C, Marra M, Savage K, Connors J, Shah S, Gascoyne R, Steidl C
    Nat Genet, 2014-02-16;46(4):329-35.
    Species: Human
    Sample Types: Whole Cells

FAQs

No product specific FAQs exist for this product, however you may

View all Activity Assay FAQs

Reviews for Human Active PTP1B DuoSet IC, 2 Plate

There are currently no reviews for this product. Be the first to review Human Active PTP1B DuoSet IC, 2 Plate and earn rewards!

Have you used Human Active PTP1B DuoSet IC, 2 Plate?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review