Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIA/ALK-2

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

References

Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Long Name

Activin Receptor IA

Entrez Gene IDs

90 (Human); 11477 (Mouse)

Alternate Names

activin A receptor, type I; Activin RIA; ActivinRIA; ACTRI; ACVR1; ACVR1A; ACVRLK2; ALK2; ALK-2; FOP; SKR1; TSRI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Activin RIA/ALK-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma.
Authors: Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J
PLoS ONE, 2012;7(10):e46117.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Inhibitory effects and target genes of bone morphogenetic protein 6 in Jurkat TAg cells.
Authors: Sivertsen EA, Huse K, Hystad ME, Kersten C, Smeland EB, Myklebust JH
Eur. J. Immunol., 2007;37(10):2937-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3.
Authors: Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E
Exp. Hematol., 2006;34(1):72-81.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry