Human ADAM32 Antibody [Biotin]

R&D Systems | Catalog # BAF5380

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all ADAM32 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Ser17-Thr476
Accession # Q8TC27

Specificity

Detects human ADAM32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) ADAM9, rhADAM22, and rhADAM23 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ADAM32 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM32

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM32

ADAM32 (a disintegrin and metalloprotease domain 32) is a 90‑100 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin-binding disintegrin region (aa 391‑479), a Cys-rich segment (aa 480 - 502) and an EGF-like domain (aa 622‑654). In the testis, mature ADAM32 is approximately 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Long Name

A Disintegrin and Metalloproteinase Domain 32

Alternate Names

a disintegrin and metalloprotease domain 32, a disintegrin and metalloproteinase domain 32, ADAM 32, ADAM metallopeptidase domain 32, disintegrin and metalloproteinase domain-containing protein 32, FLJ26299, FLJ29004, metalloproteinase 12-like protein

Entrez Gene IDs

203102 (Human); 353188 (Mouse); 361170 (Rat)

Gene Symbol

ADAM32

UniProt

Q8TC27

Additional ADAM32 Products

Product Documents for Human ADAM32 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM32 Antibody [Biotin]

For research use only

Related Research Areas

Metalloproteases and Regulators

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