Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAM32 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Ser17-Thr476
Accession # Q8TC27

Specificity

Detects human ADAM32 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ADAM9, rhADAM22, and rhADAM23 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ADAM32 Antibody

Detection of Human ADAM32 antibody by Western Blot.

Detection of Human ADAM32 by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ADAM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAM32 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human ADAM32 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM32, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM32

ADAM32 (a disintegrin and metalloprotease domain 32) is a 90‑100 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin-binding disintegrin region (aa 391‑479), a Cys-rich segment (aa 480 - 502) and an EGF-like domain (aa 622‑654). In the testis, mature ADAM32 is approximately 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Long Name

A Disintegrin and Metalloproteinase Domain 32

Alternate Names

a disintegrin and metalloprotease domain 32, a disintegrin and metalloproteinase domain 32, ADAM 32, ADAM metallopeptidase domain 32, disintegrin and metalloproteinase domain-containing protein 32, FLJ26299, FLJ29004, metalloproteinase 12-like protein

Entrez Gene IDs

203102 (Human); 353188 (Mouse); 361170 (Rat)

Gene Symbol

ADAM32

UniProt

Q8TC27

Additional ADAM32 Products

Product Documents for Human ADAM32 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAM32 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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