Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all ADAM8 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM8
Glu158-Ser653
Accession # P78325

Specificity

Detects human ADAM8 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) ADAM15, recombinant mouse (rm) ADAM9, rmADAM10, rhBACE and rhTACE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ADAM8 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References

  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Alternate Names

CD156a, MS2

Entrez Gene IDs

101 (Human)

Gene Symbol

ADAM8

UniProt

P78325

Additional ADAM8 Products

Product Documents for Human ADAM8 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM8 Biotinylated Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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