Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Native SDS-PAGE

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ADAM8 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM8 Ectodomain
Glu158-Ser653
Accession # P78325

Specificity

Detects human ADAM8 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse ADAM8 is observed and less than 1% cross-reactivity with recombinant human (rh) ADAM12, rhADAM19, and rhADAM33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ADAM8 Ectodomain Antibody

ADAM8 antibody in Human PBMCs by Immunocytochemistry (ICC).

ADAM8 in Human PBMCs.

ADAM8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human ADAM8 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1031) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human ADAM8 Ectodomain Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD)

Reviewed Applications

Read 3 reviews rated 4.3 using AF1031 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References

  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Alternate Names

CD156a, MS2

Entrez Gene IDs

101 (Human)

Gene Symbol

ADAM8

UniProt

P78325

Additional ADAM8 Products

Product Documents for Human ADAM8 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAM8 Ectodomain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAM8 Ectodomain Antibody

Customer Reviews for Human ADAM8 Ectodomain Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Human ADAM8 Ectodomain Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 06/23/2017
    Human ADAM8 Ectodomain Antibody AF1031
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Human intervetebral disc nucleus pulposus
    Species: Human
    Verified Customer | Posted 01/27/2015
    Human nucleus pulposus ADAM8 IHC
    Human ADAM8 Ectodomain Antibody AF1031
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23101757
    Species: Human
    Verified Customer | Posted 01/05/2015

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Protocols

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