Human ADAMTS8 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF6614T Datasheet / COA / SDS

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AF6614T-100UG

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Human ADAMTS8 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS8 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS8
Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala)
Accession # AAD48081

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAMTS8

ADAMTS8 (A disintegrin and metalloprotease with thrombospondin motifs 8; also METH-2) is a 95 kDa member of an ADAMTS subfamily of Zn metalloproteases that includes ADAMST-1, -4, -5 and -15. It is expressed by chrondrocytes, neurons, astrocytes and macrophages, and likely participates in proteoglycan (aggrecan) proteolysis. Human proADAMTS8 is a secreted, 863 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 27-213), a peptidase M12B domain (aa 219-429), a disintegrin region (aa 438-525), and two TSP type I sequences (aa 526-888) that are separated by an intervening spacer domain (aa 690-831). Cleavage of the proregion generates a mature 80 kDa molecule that may undergo additional processing to create a 65-67 kDa truncated form. There are two potential splice variants. One shows a 31 aa substitution for aa 411-889, while another shows an alternative start site at Met231. Over aa 29-691, human ADAMTS8 shares 79% aa identity with mouse ADAMTS8.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 8

Entrez Gene IDs

11095 (Human); 30806 (Mouse); 300475 (Rat)

Alternate Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 8; ADAM metallopeptidase with thrombospondin type 1 motif, 8; ADAMTS8; ADAMTS-8; ADAM-TS8A disintegrin and metalloproteinase with thrombospondin motifs 8; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82; FLJ41712; METH-2; METH2ADAM-TS 8; METH-8