Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAMTS8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS8
Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala)
Accession # AAD48081

Specificity

Detects human ADAMTS8 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ADAMTS8 Antibody

Detection of Human ADAMTS8 antibody by Western Blot.

Detection of Human ADAMTS8 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector. PVDF Membrane was probed with 1 µg/mL of Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS8 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ADAMTS8 antibody in Human Brain by Immunohistochemistry (IHC-P).

ADAMTS8 in Human Brain.

ADAMTS8 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ADAMTS8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS8, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS8

ADAMTS8 (A disintegrin and metalloprotease with thrombospondin motifs 8; also METH-2) is a 95 kDa member of an ADAMTS subfamily of Zn metalloproteases that includes ADAMST-1, -4, -5 and -15. It is expressed by chrondrocytes, neurons, astrocytes and macrophages, and likely participates in proteoglycan (aggrecan) proteolysis. Human proADAMTS8 is a secreted, 863 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 27-213), a peptidase M12B domain (aa 219-429), a disintegrin region (aa 438-525), and two TSP type I sequences (aa 526-888) that are separated by an intervening spacer domain (aa 690-831). Cleavage of the proregion generates a mature 80 kDa molecule that may undergo additional processing to create a 65-67 kDa truncated form. There are two potential splice variants. One shows a 31 aa substitution for aa 411-889, while another shows an alternative start site at Met231. Over aa 29-691, human ADAMTS8 shares 79% aa identity with mouse ADAMTS8.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 8

Alternate Names

METH-2, METH-8

Entrez Gene IDs

11095 (Human); 30806 (Mouse); 300475 (Rat)

Gene Symbol

ADAMTS8

UniProt

AAD48081

Additional ADAMTS8 Products

Product Documents for Human ADAMTS8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAMTS8 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAMTS8 Antibody

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Protocols

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