Human AG-2/AGR2 Antibody

Detection of gy8g5kpfalnhjsugszqt.jpg by Immunohistochemistry

Decreased mucus expression and increased methylation in the Piezo1 flox-mucin2 Cre mice. A Immunohistochemistry of Piezo1 in WT and Piezo1 flox-mucin2 Cre mice (KO mice); bar length: 20 μm. B Western blot of Piezo1 in WT and KO mice. C, D Alcian staining and statistical analysis of mucus thickness in WT and KO mice (n ≥ 6); bar length: 50 μm. E RT-PCR of mucin2 in WT and KO mice (n ≥ 6). F, G Immunofluorescence and statistical analysis of goblet cells/ epithelial cells in WT and KO mice (n ≥ 6); bar length: 20 μm. H FISH of WT and KO mice, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 20 μm. I–J Western blot and statistical analysis of SUV39h1, Hdac3 and H3K9me3 in the colon mucosa of WT and KO mice. K Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of WT and KO mice; bar length: 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of hfx63h772x3b3pha9mga.jpg by Immunohistochemistry

Expression of Piezo1 in colon. (A) Expression of Piezo1 in colon. (B) Relative mRNA expression of Piezo1. (C) The co-localization of AGR2 and GC Piezo1 in colon (400×). Data are expressed as the mean ± SEM (n =4-5). *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36425784), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of hqhzy4vxxzxnptablo9s.jpg by Immunohistochemistry

Piezo1 agonist Yoda1 reduced the methylation of intestinal mucosa in WAS model mice. A Immunofluorescence for co-localization of AGR2 and H3K9me3 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. B Immunofluorescence for co-localization of AGR2 and Piezo1 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. C Western blot of Piezo1, Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 in the control and WAS mice (n ≥ 6). D Statistical analysis of protein in panel C. E RT-PCR of Piezo1, EDEM1, GRP78, ATF4 mRNA in the mucosa of control, WAS and WAS + Yoda1 mice (n ≥ 6). F Schematic showed that Yoda1 reduced the H3K9me3 modification on the mucin2 promoter by reducing Hdac3 and SUV39h1 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Decreased mucus expression and increased methylation in the Piezo1 flox-mucin2 Cre mice. A Immunohistochemistry of Piezo1 in WT and Piezo1 flox-mucin2 Cre mice (KO mice); bar length: 20 μm. B Western blot of Piezo1 in WT and KO mice. C, D Alcian staining and statistical analysis of mucus thickness in WT and KO mice (n ≥ 6); bar length: 50 μm. E RT-PCR of mucin2 in WT and KO mice (n ≥ 6). F, G Immunofluorescence and statistical analysis of goblet cells/ epithelial cells in WT and KO mice (n ≥ 6); bar length: 20 μm. H FISH of WT and KO mice, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 20 μm. I–J Western blot and statistical analysis of SUV39h1, Hdac3 and H3K9me3 in the colon mucosa of WT and KO mice. K Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of WT and KO mice; bar length: 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunocytochemistry/ Immunofluorescence

Decreased self-renewal capacity of colon stem cells from Piezo1 delta GC mice. (A) Representative images of colonoids from WT and Piezo1 delta GC mice at day 5. Images of one colonoid on day 1, 3, and 5 are below, reflecting the growth process of a colonoid. Scale bar: 100 μm. (B–D) Indicators related to colonoids growth: number of buds per colonoid (B), surface area per colonoid (C), and percentage of colonoids with buds per well (D). (E) Immunofluorescence staining of EDU and Ki67 in colonoids from WT and Piezo1 delta GC mice. Scale bar: 100 μm. (F) Statistical analysis of EDU and Ki67 positive cell ratio in (E). (G) Immunofluorescence staining of differentiated colonocyte (Alpi), goblet cell (Agr2), and enteroendocrine cell (Chga) in colonoids from WT and Piezo1 delta GC mice. Scale bar: 100 μm. (H) Statistical analysis of Alpi, Agr2, and Chga positive cell ratio in (G). (I) RNA levels of Ki67 from WT and Piezo1 delta GC mouse colonoids (normalized to GAPDH). (J) AB-PAS staining of colonoids from WT and Piezo1 delta GC mice. The red arrows indicate mucus secreted by goblet cells in colonoids. Scale bar: 100 μm. At least three independent experiments were conducted. Data are presented as the mean ± SEM. (n = 5 mice). ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37762681), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Identification of Piezo1 delta GC mice. (A) Immunofluorescence co-localization of Piezo1 and Agr2 (label goblet cells) in WT and Piezo1 delta GC mouse colons. The yellow box shows a magnified localized image. Scale bar: 100 μm. (B) mRNA level of Piezo1 in WT and Piezo1 delta GC mouse colon crypts (normalized to GAPDH). At least three independent experiments were conducted. Data are expressed as the mean ± SEM. (n = 5 mice). ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37762681), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Decreased Piezo1 and enhanced methylation in the intestinal mucosa of WAS model mice. A Western blot of Piezo1, Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 (Internal parameter for nuclear protein H3K9me3) in the mucosa of control and WAS mice. B Statistical analysis of protein expression in panel A. C Immunofluorescence for co-localization of AGR2 (label of goblet cells) and H3K9me3 in intestinal mucosa of control and WAS mice; bar length: 20 μm. D Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of control and WAS mice; bar length: 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Decreased Piezo1 and enhanced methylation in the intestinal mucosa of WAS model mice. A Western blot of Piezo1, Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 (Internal parameter for nuclear protein H3K9me3) in the mucosa of control and WAS mice. B Statistical analysis of protein expression in panel A. C Immunofluorescence for co-localization of AGR2 (label of goblet cells) and H3K9me3 in intestinal mucosa of control and WAS mice; bar length: 20 μm. D Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of control and WAS mice; bar length: 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Piezo1 agonist Yoda1 reduced the methylation of intestinal mucosa in WAS model mice. A Immunofluorescence for co-localization of AGR2 and H3K9me3 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. B Immunofluorescence for co-localization of AGR2 and Piezo1 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. C Western blot of Piezo1, Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 in the control and WAS mice (n ≥ 6). D Statistical analysis of protein in panel C. E RT-PCR of Piezo1, EDEM1, GRP78, ATF4 mRNA in the mucosa of control, WAS and WAS + Yoda1 mice (n ≥ 6). F Schematic showed that Yoda1 reduced the H3K9me3 modification on the mucin2 promoter by reducing Hdac3 and SUV39h1 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Decreased GC numbers and weakened mucus layer in GC Piezo1-/- mice. (A) The mucus layer in colon tissue by AB-PAS staining. 200×. (B) the number of goblet cells and the thickness of the inner mucus layer from two groups. (C) The typical immunofluorescent images of colon tissues were stained with DAPI (blue) and Mucin2 (red) or AGR2 (red) and were observed using a confocal laser-scanning microscope. 400×. (D) The relative mean fluorescence intensity of AGR2, Mucin2. (E) The relative mRNA expression of Mucin2, AGR2, and TFF3 in colonic tissue. (F) The relative mRNA expression of DOCK4, Hes1, Spdef, and Klf4 in colonic tissue. Data are expressed as the mean ± SEM. (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001;. ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36425784), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Decreased mucus expression and increased methylation in the Piezo1 flox-mucin2 Cre mice. A Immunohistochemistry of Piezo1 in WT and Piezo1 flox-mucin2 Cre mice (KO mice); bar length: 20 μm. B Western blot of Piezo1 in WT and KO mice. C, D Alcian staining and statistical analysis of mucus thickness in WT and KO mice (n ≥ 6); bar length: 50 μm. E RT-PCR of mucin2 in WT and KO mice (n ≥ 6). F, G Immunofluorescence and statistical analysis of goblet cells/ epithelial cells in WT and KO mice (n ≥ 6); bar length: 20 μm. H FISH of WT and KO mice, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 20 μm. I–J Western blot and statistical analysis of SUV39h1, Hdac3 and H3K9me3 in the colon mucosa of WT and KO mice. K Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of WT and KO mice; bar length: 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Expression of Piezo1 in colon. (A) Expression of Piezo1 in colon. (B) Relative mRNA expression of Piezo1. (C) The co-localization of AGR2 and GC Piezo1 in colon (400×). Data are expressed as the mean ± SEM (n =4-5). *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36425784), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of AG-2/AGR2 by Immunohistochemistry

Piezo1 agonist Yoda1 reduced the methylation of intestinal mucosa in WAS model mice. A Immunofluorescence for co-localization of AGR2 and H3K9me3 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. B Immunofluorescence for co-localization of AGR2 and Piezo1 in intestinal mucosa of control, WAS and WAS + Yoda1 mice; bar length: 20 μm. C Western blot of Piezo1, Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 in the control and WAS mice (n ≥ 6). D Statistical analysis of protein in panel C. E RT-PCR of Piezo1, EDEM1, GRP78, ATF4 mRNA in the mucosa of control, WAS and WAS + Yoda1 mice (n ≥ 6). F Schematic showed that Yoda1 reduced the H3K9me3 modification on the mucin2 promoter by reducing Hdac3 and SUV39h1 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36631841), licensed under a CC-BY license. Not internally tested by R&D Systems.