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Human AG-3 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF6307U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6307U-100UG

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Human AG-3 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human AG‑2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E.coli-derived Recombinant Human AG-3
Ile22-Leu166
Accession # Q8TD06
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AG-3

AG3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG3 is presumably secreted and binds to both a-dystroglycan and C4.4a/LYPD3. Mature human AG3 is 145 amino acids (aa) in length (aa 22‑166) (SwissProt #:Q8TD06). Over aa 22-166, human AG3 shares 92% amino acid sequence identity with mouse AG3.

Long Name
Anterior Gradient 3 Homolog
Entrez Gene IDs
155465 (Human); 403205 (Mouse); 298959 (Rat)
Alternate Names
AG3; AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18

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