Human AG-3 Antibody

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Detection of Human AG‑3 by Western Blot.
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Product Details
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Human AG-3 Antibody Summary

Species Reactivity
Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human AG‑2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E.coli-derived Recombinant Human AG-3
Accession # Q8TD06
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human AG‑3 antibody by Western Blot. View Larger

Detection of Human AG‑3 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Human AG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG-3 at approximately 19kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry AG-3 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

AG‑3 in Human Breast. AG-3 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human AG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AG-3

AG3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG3 is presumably secreted and binds to both a-dystroglycan and C4.4a/LYPD3. Mature human AG3 is 145 amino acids (aa) in length (aa 22‑166) (SwissProt #:Q8TD06). Over aa 22-166, human AG3 shares 92% amino acid sequence identity with mouse AG3.

Long Name
Anterior Gradient 3 Homolog
Entrez Gene IDs
155465 (Human); 403205 (Mouse); 298959 (Rat)
Alternate Names
AG3; AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18

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