Human AgRP/ART Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # AF704AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG
View all AgRP/ART Primary Antibodies »

Product Specifications

Specificity

Detects human AgRP/ART in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP/ART is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human AgRP/ART Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AgRP/ART

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).

References

References
  1. Martin, N.M. et al. (2006) Peptides 27:333.
  2. Fan, W. et al. (1997) Nature 385:165.
  3. Ollmann, M.M. et al. (1997) Science 278:135.
  4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
  5. Shutter, J.R. et al. (1997) Genes Develop. 11:593.
  6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
  7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
  8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
  9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
  10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
  11. Small, C.K. et al. (2001) Diabetes 50:248.
  12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
  13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
  14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265 - 266:108.

Long Name

Agouti-related Protein

Alternate Names

ART

Entrez Gene IDs

181 (Human); 11604 (Mouse)

Gene Symbol

AGRP

UniProt

O00253

Additional AgRP/ART Products

Product Documents for Human AgRP/ART Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human AgRP/ART Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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