Human alpha-Fetoprotein/AFP Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1369G-100UG
Detection  of  alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry.
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Human alpha-Fetoprotein/AFP Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Fetoprotein in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 189506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human umbilical cord serum-derived alpha -Fetoprotein
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of  alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # IC1369G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.

References
  1. Matsumura, M. et al. (2001) Hepatol. Res. 20:84
  2. Deutsch, H.F. et al. (2000) Tumor Biol. 21:267
    Entrez Gene IDs
    174 (Human); 11576 (Mouse); 24177 (Rat)
    Alternate Names
    AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP

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