Human alpha -Fetoprotein/AFP Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human alpha -Fetoprotein in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 189506
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human umbilical cord serum-derived alpha -Fetoprotein
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.
alpha ‑Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human
alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
AFP ( alpha -Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.
  • References:
    1. Matsumura, M. et al. (2001) Hepatol. Res. 20:84
    2. Deutsch, H.F. et al. (2000) Tumor Biol. 21:267
    3. Entrez Gene IDs:
      174 (Human); 11576 (Mouse); 24177 (Rat)
    4. Alternate Names:
      AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
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    Species
    Applications
    Sample Type
    1. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
      PLoS ONE, 2016;11(9):e0161229.
      Species: Human
      Sample Type: Whole Cells
      Application: IHC Not Specified
    2. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
      Nat Commun, 2016;7(0):13602.
      Species: Human
      Sample Type: Cell Culture Supernates
      Application: WB
    3. Frontotemporal dementia-associated N279K tau mutant disrupts subcellular vesicle trafficking and induces cellular stress in iPSC-derived neural stem cells.
      Authors: Wren M, Zhao J, Liu C, Murray M, Atagi Y, Davis M, Fu Y, Okano H, Ogaki K, Strongosky A, Tacik P, Rademakers R, Ross O, Dickson D, Wszolek Z, Kanekiyo T, Bu G
      Mol Neurodegener, 2015;10(0):46.
      Species: Human
      Sample Type: Whole Cells
      Application: IHC Not Specified
    4. Embryoid body formation of human amniotic fluid stem cells depends on mTOR.
      Authors: Valli A, Rosner M, Fuchs C, Siegel N, Bishop CE, Dolznig H, Madel U, Feichtinger W, Atala A, Hengstschlager M
      Oncogene, 2010;29(7):966-77.
      Species: Human
      Sample Type: Whole Cells
      Application: ICC
    5. Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway.
      Authors: Vallier L, Reynolds D, Pedersen RA
      Dev. Biol., 2004;275(2):403-21.
      Species: Human
      Sample Type: Whole Cells
      Application: ICC
    6. Central role of complement membrane attack complex in monosodium urate crystal-induced neutrophilic rabbit knee synovitis.
      Authors: Tramontini N, Huber C, Liu-Bryan R, Terkeltaub RA, Kilgore KS
      Arthritis Rheum., 2004;50(8):2633-9.
      Species: Human
      Sample Type: Whole Cells
      Application: ICC
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