Human alpha -Fetoprotein/AFP Antibody

Catalog # Availability Size / Price Qty
MAB1369
MAB1369-SP
alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.
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Product Details
Citations (7)
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Human alpha -Fetoprotein/AFP Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Fetoprotein in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 189506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human umbilical cord serum-derived alpha -Fetoprotein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
alpha -Fetoprotein/AFP was specifically detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in alpha -Fetoprotein/AFP knockout (KO) HepG2 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. View Larger

alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.
alpha ‑Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human
alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection  of  alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Knockout Validated alpha ‑Fetoprotein/AFP  Specificity is Shown by Immunocytochemistry in Knockout Cell Line. View Larger

alpha ‑Fetoprotein/AFP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in alpha ‑Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Mouse Anti-Human alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.

References
  1. Matsumura, M. et al. (2001) Hepatol. Res. 20:84
  2. Deutsch, H.F. et al. (2000) Tumor Biol. 21:267
    Entrez Gene IDs
    174 (Human); 11576 (Mouse); 24177 (Rat)
    Alternate Names
    AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP

    Product Datasheets

    Citations for Human alpha -Fetoprotein/AFP Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    7 Citations: Showing 1 - 7
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    1. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells
      Authors: J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman
      Mol Autism, 2018;9(0):44.
      Species: Human
      Sample Types: organoid
      Applications: IHC
    2. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
      Nat Commun, 2016;7(0):13602.
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: WB
    3. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
      PLoS ONE, 2016;11(9):e0161229.
      Species: Human
      Sample Types: Whole Cells
      Applications: IHC Not Specified
    4. Frontotemporal dementia-associated N279K tau mutant disrupts subcellular vesicle trafficking and induces cellular stress in iPSC-derived neural stem cells.
      Authors: Wren M, Zhao J, Liu C, Murray M, Atagi Y, Davis M, Fu Y, Okano H, Ogaki K, Strongosky A, Tacik P, Rademakers R, Ross O, Dickson D, Wszolek Z, Kanekiyo T, Bu G
      Mol Neurodegener, 2015;10(0):46.
      Species: Human
      Sample Types: Whole Cells
      Applications: IHC Not Specified
    5. Embryoid body formation of human amniotic fluid stem cells depends on mTOR.
      Authors: Valli A, Rosner M, Fuchs C, Siegel N, Bishop CE, Dolznig H, Madel U, Feichtinger W, Atala A, Hengstschlager M
      Oncogene, 2010;29(7):966-77.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    6. Central role of complement membrane attack complex in monosodium urate crystal-induced neutrophilic rabbit knee synovitis.
      Authors: Tramontini N, Huber C, Liu-Bryan R, Terkeltaub RA, Kilgore KS
      Arthritis Rheum., 2004;50(8):2633-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    7. Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway.
      Authors: Vallier L, Reynolds D, Pedersen RA
      Dev. Biol., 2004;275(2):403-21.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC

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    Cell and Tissue Staining Kits

    Isotype Controls

    Reconstitution Buffers

    Secondary Antibodies

    Mouse IgG2B Antibody

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