Human AMIGO2 Antibody

Name: Human AMIGO2 Antibody
Brand: R&D Systems
SKU: MAB20801

Catalog #: MAB20801 Datasheet / COA / SDS

Discontinued Product

MAB20801 has been discontinued.

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Human AMIGO2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human AMIGO2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human AMIGO1 or recombinant mouse AMIGO2 is observed.

Source

Monoclonal Mouse IgG₃ Clone # 273610

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human AMIGO2
Gly39-Ala392
Accession # Q86SJ2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human AMIGO2

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

Long Name

Amphoterin-induced Gene and ORF 2

Entrez Gene IDs

347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)

Alternate Names

adhesion molecule with Ig-like domain 2; ALI1amphoterin induced gene 2; alivin 1; Alivin-1; AMIGO2; AMIGO-2; DEGAamphoterin-induced protein 2; differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; transmembrane protein AMIGO2