Human Amnionless Biotinylated Antibody

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BAF1860
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Human Amnionless Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Amnionless in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Amnionless
Val20-Ala358
Accession # Q9BXJ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Amnionless (Catalog # 1860-AM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amnionless

Amnionless (AMN) is an approximately 48 kDa type I transmembrane protein that is required for surface expression of the extracellular membrane-associated protein Cubilin (1‑5). The two form a complex termed CUBAM (4, 5). The 453 amino acid (aa) human AMN precursor contains a 19 aa signal sequence, a 338 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 75 aa cytoplasmic domain. The ECD contains two potential N-glycosylation sites and a cysteine-rich vWFC domain. Two cytoplasmic consensus FxNPxP/F sequences for clathrin-coated pit targeting mediate endocytosis of compounds bound by Cubulin (3, 5). The ECD of human AMN shares 67%, 66%, 76% and 78% aa identity with mouse, rat, canine and bovine AMN, respectively. AMN is present during gastrulation in the visceral endoderm and required for primitive streak formation during embryonic development in mouse (6). Transcription from an alternate start site in humans results in an isoform starting at M55 that may also function in development (7). AMN is highly expressed on polarized epithelia in the apical brush border membranes of small intestine and kidney proximal convoluted tubules (3‑5, 7). Intestinal CUBAM is required for absorption of cobalamin (vitamin B12) when complexed with intrinsic factor (IF), and mutations of AMN or Cubilin cause Imersund-Grasbeck syndrome, also called megaloblastic anemia-1 (4, 5, 7). In the kidney, the CUBAM complex is thought to be important for reabsorption of proteins from filtrate, notably albumin and the molecules it carries (3, 8). Cubilin or CUBAM can be tightly associated with megalin, another endocytic receptor that contributes to stable expression of Cubilin, and to uptake of vitamin and lipoprotein complexes in the kidney and placenta (1, 9).

References
  1. Kozyraki, R. and F. Gofflot (2007) Curr. Pharm. Des. 13:3038.
  2. Coudroy, G. et al. (2005) J. Am. Soc. Nephrol. 16:2330.
  3. Strope, S. et al. (2004) Development 131:4787.
  4. He, Q. et al. (2005) Blood 106:1447.
  5. Fyfe, J.C. et al. (2004) Blood 103:1573.
  6. Kalantry, S. et al. (2001) Nat. Genet. 27:412.
  7. Tanner, S.M. et al. (2003) Nat. Genet. 33:426.
  8. Birn, H. (2006) Am. J. Physiol. Renal Physiol. 291:F22.
  9. Ahuja, R. et al. (2008) Biochem. J. 410:301.
Entrez Gene IDs
81693 (Human); 93835 (Mouse)
Alternate Names
AMN; amnionless homolog (mouse); Amnionless; PRO1028; protein amnionless; UNQ513/PRO1028; visceral endoderm-specific type 1 transmembrane protein

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