Human Apolipoprotein A-I Quantikine ELISA Kit

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DAPA10
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Human Apolipoprotein A-I/ApoA1 Standard Curve
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Human Apolipoprotein A-I Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
Sensitivity
0.603 ng/mL
Assay Range
6.3 - 200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human ApoA1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Apolipoprotein A-I/ApoA1 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human ApoA1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human ApoA1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ApoA1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ApoA1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 30.3 60.2 120 35.5 67.9 129
Standard Deviation 1.43 2.17 4.05 1.92 4.65 9.82
CV% 4.7 3.6 3.4 5.4 6.8 7.6

Recovery

The recovery of human ApoA1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 90-114
Urine (n=4) 99 83-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ApoA1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Apolipoprotein A-I/ApoA1 ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Apolipoprotein A-I/ApoA1

The apolipoproteins are a structurally-unrelated group of proteins that have some association with the transport of lipids in blood. Apolipoproteins, plus phospholipids, cholesterol and triglycerides, form spherical particles with a lipid/hydrophobic center and a (apolipo)protein coat. The apolipoprotein coat promotes aqueous solubility and serves as a ligand for lipoprotein receptors. HDL may contain apolipoproteins A, C, D, E, J, L and M, while LDL contains apolipoproteins B and E.

ApoAI and ApoA2 are major protein components of serum high-density lipoprotein (HDL) and are produced by the liver and small intestine. They are involved in reverse cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 aa in length. It binds LDL to the ApoB/E receptor. ApoC activates lipoprotein lipase and may self-associate to form amyloid-type fibrils.

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders, particularly Alzheimer's disease.

Serum amyloid A proteins (SAAs) are a family of homologous apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group consists of the acute phase SAA1 and SAA2 that associate with HDL during inflammation and remodel the HDL particle by displacing apolipoprotein A1. The second group consists of constitutively expressed SAA4 and SAA5 that exist as minor apolipoproteins on HDL but make up more than 90% of the total SAA during homeostasis.

Entrez Gene IDs:
335 (Human); 11806 (Mouse); 25081 (Rat)
Alternate Names:
Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for Human Apolipoprotein A-I Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MicroRNA-33 regulates the population of peripheral inflammatory Ly6Chigh monocytes through dual pathways
    Authors: O Baba, T Horie, T Nakao, D Hakuno, Y Nakashima, H Nishi, Y Kuwabara, M Nishiga, T Nishino, Y Ide, F Nakazeki, S Koyama, M Kimura, R Hanada, M Kawahara, T Kimura, K Ono
    Mol. Cell. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum

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