Human Arginase 2/ARG2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10602-100
MAB10602-SP
Detection of Human Arginase 2/ARG2 by Western Blot.
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Human Arginase 2/ARG2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Arginase 2/ARG2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2611B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived human Arginase 2/ARG2
Val23-Ile354
Accession # P78540
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
HepG2 human hepatocellular carcinoma cell line
Simple Western
5 µg/mL
LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Arginase 2/ARG2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human Arginase 2/ARG2 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arginase 2/ARG2

Arginase-2, a binuclear manganese-dependent metalloenzyme, is an approximately 36 kDa protein that forms a trimeric active molecule that catalyses the conversion of L-arginine into L-ornithine and urea (1). There are two isoforms of human arginase that share 58% sequence identity. Arginases are critical to the urea cycle and also influence downstream nitric oxide and polyamine levels (2) and hence are implicated to play a role in a number of diseases. While Arginase 1 is cytosolic and expressed predominantly in the liver, Arginase 2 is a mitochondrial enzyme expressed in several non-hepatic tissues including kidney, prostate and immune cells where it is regulated by inflammatory cytokines (2,3). Arginase 2 is implicated in regulation of a nitric oxide-dependent inflammatory response in lung and bronchial epithelial disease (4,5).  It has been implicated in neuronal disease such as Huntington's disease due to nitric oxide signaling and suppressed enzymatic activity (6) and to play a role in atherosclerosis and hypertension through signaling pathways independent of its enzymatic activity (7,8). Arginase 2 promotes tumorigenesis through immune suppression, metabolism, cell proliferation and vascularization (9-11). Arginase-2 was noted as an attractive tumor therapeutic target (10) given that genetic deletion in mice does not lead to significant abnormalities (12).

References
  1. Dowling, D. et al. (2008) Cell Mol. Life Sci. 65:2039.
  2. Munder, M. (2009) Br. J. Pharmacol. 158:638.
  3. Geiger, R. et al. (2016) Cell 167:829.
  4. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
  5. Xu, W. et al. (2016) J. Clin. Invest. 126:2465.
  6. Bichell, T.J.V. et al. (2017) Biochim. Biophys. Acta 1863:1596.
  7. Jin, Y. et al. (2014) Am. J. Physiol. Lung Cell Mol. Physiol. 307:L197.
  8. Xiong, Y. et al. (2014) Autophagy 10:2223.
  9. Ino, Y. et al. (2013) PLoS One 8:e55146.
  10. Zaytouni, T. et al. (2017) Nat. Commun. 8:242.
  11. Costa, H. et al. (2016) Oncotarget 7:47221.
  12. Shi, O. et al. (2001) Mol. Cell Biol. 21:811.
Long Name
Kidney-Type Arginase
Entrez Gene IDs
384 (Human); 11847 (Mouse); 29215 (Rat)
Alternate Names
ARG2; Arginase 2; arginase, type II; Arginase-2; arginase-2, mitochondrial; EC 3.5.3; EC 3.5.3.1; kidney arginase; Kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; Non-hepatic arginase; Type II Arginase

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