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Human ATP5A1 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB11558U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB11558U-100UG

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Human ATP5A1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects a synthetic peptide around aa 235 in Direct ELISA
Source
Monoclonal Mouse IgG2b Clone # 1082626
Purification
Protein A or G purified
Immunogen
ATP5A1 contatining synthetic peptide
Accession # P25705
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ATP5A1

ATP5F1A is a 553aa, 60kDa protein which encodes subunit alpha of mitochondrial ATP synthase (complex V), the rotary ATPase responsible for more than 90% of cellular ATP synthesis.  F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. ATP5A1 is generally regarded to mediate tumor progression through mitochondrial signaling.  It is overrepresented in an oxidative phosphorylation pathway associated with tumorigenesis and tumor progression. Dysregulation of this ATP synthase subunit is observed in human carcinomas.

Long Name
ATP5A1
Entrez Gene IDs
498 (Human); 11946 (Mouse); 65262 (Rat)
Alternate Names
ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 co; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5A1; ATP5AL2; ATP5AMOM2; ATPM; ATPMATP synthase subunit alpha, mitochondrial; cardiac muscle; COXPD22; EC 3.6.3; EC 3.6.3.14; epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; MC5DN4A; MC5DN4B; mitochondrial ATP synthetase, oligomycin-resistant; MOM2; OMR; ORM; ORMATP synthase alpha chain, mitochondrial

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