Human ATP5A1 Antibody

Catalog #: MAB11558 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11558-100
MAB11558-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ATP5A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide around aa 235 in Direct ELISA

Source

Monoclonal Mouse IgG_2B Clone # 1082626

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

ATP5A1 contatining synthetic peptide
Accession # P25705

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human liver

Immunocytochemistry

3-25 µg/mL

Formalin fixed HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of ATP5A1 in Human Liver. ATP5A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ATP5A1 Monoclonal Antibody (Catalog # MAB11558) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of mitochondria. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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Detection of ATP5A1 in HepG2 Human Cell Line. ATP5A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ATP5A1 Monoclonal Antibody (Catalog # MAB11558) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the membrane of mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATP5A1

ATP5F1A is a 553aa, 60kDa protein which encodes subunit alpha of mitochondrial ATP synthase (complex V), the rotary ATPase responsible for more than 90% of cellular ATP synthesis. F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. ATP5A1 is generally regarded to mediate tumor progression through mitochondrial signaling. It is overrepresented in an oxidative phosphorylation pathway associated with tumorigenesis and tumor progression. Dysregulation of this ATP synthase subunit is observed in human carcinomas.

References

Lines MA, Cuillerier A, Chakraborty P, Naas T, Duque Lasio ML, Michaud J, Pileggi C, Harper ME, Burelle Y, Toler TL, Sondheimer N, Crawford HP, Millan F, Geraghty MT. A recurrent de novo ATP5F1A substitution associated with neonatal complex V deficiency. Eur J Hum Genet. 2021 Nov;29(11):1719-1724. doi: 10.1038/s41431-021-00956-0. Epub 2021 Sep 6. PMID: 34483339; PMCID: PMC8560863.
Song Ba Y, Wang Ma F, Wei Ma Y, Chen Ba D, Deng Ba G. ATP5A1 Participates in Transcriptional and Posttranscriptional Regulation of Cancer-Associated Genes by Modulating Their Expression and Alternative Splicing Profiles in HeLa Cells. Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211039126. doi: 10.1177/15330338211039126. PMID: 34520292; PMCID: PMC8445539.
Yuan L, Chen L, Qian K, Wang G, Lu M, Qian G, Cao X, Jiang W, Xiao Y, Wang X. A novel correlation between ATP5A1 gene expression and progression of human clear cell renal cell carcinoma identified by co‑expression analysis. Oncol Rep. 2018 Feb;39(2):525-536. doi: 10.3892/or.2017.6132. Epub 2017 Dec 4. PMID: 29207195; PMCID: PMC5783621.

Long Name

ATP5A1

Entrez Gene IDs

498 (Human); 11946 (Mouse); 65262 (Rat)

Alternate Names

ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 co; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5A1; ATP5AL2; ATP5AMOM2; ATPM; ATPMATP synthase subunit alpha, mitochondrial; cardiac muscle; COXPD22; EC 3.6.3; EC 3.6.3.14; epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; MC5DN4A; MC5DN4B; mitochondrial ATP synthetase, oligomycin-resistant; MOM2; OMR; ORM; ORMATP synthase alpha chain, mitochondrial