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Human Atrial Natriuretic Peptide/ANP Antibody

Catalog #: MAB3366
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB3366 has been discontinued.
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Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Atrial Natriuretic Peptide/ANP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Atrial Natriuretic Peptide/ANP in direct ELISAs and Western blots. Does not detect ANF.
Source
Monoclonal Rat IgG2B Clone # 361101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Atrial Natriuretic Peptide/ANP
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ANP
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human prostate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Atrial Natriuretic Peptide/ANP

Atrial Natriuretic Peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26‑92) and atrial natriuretic factor (ANF; aa 124‑151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP‑coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

Entrez Gene IDs
4878 (Human); 230899 (Mouse); 24602 (Rat)
Alternate Names
ANF; ANP; ANPatriopeptin; ATFB6; atrial natriuretic factor; Atrial Natriuretic Peptide; Cardionatrin; CDD-ANF; natriuretic peptide A; natriuretic peptide precursor A; NPPA; PND; PNDcardionatrin; prepronatriodilatin

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Citation for Human Atrial Natriuretic Peptide/ANP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
    Authors: Yasuo T, Kitaya K
    Fertil. Steril., 2008-08-09;92(2):709-21.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot

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