This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Attractin/ATRN, also known as DPPT-L, is an approximately 200 kDa transmembrane glycoprotein that shows dipeptidyl peptidase activity similar to DPPIV/CD26. Attractin is involved in a variety of processes including monocyte-T cell adhesion, axon myelination, melanocyte pigment synthesis, and energy homeostasis. Attractin is transiently upregulated during T cell activation before expression switches to the 175 kDa secreted isoform which is released into the circulation. Soluble Attractin is preferentially expressed by leukocytes and differentiating neurons. It blocks neurite formation and is elevated in the CSF of astrocytoma patients.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.