Human B7-H6 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human B7-H6 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 875001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human B7-H6
    Accession # Q68D85
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑H6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7‑H6 APC‑conjugated Monoclonal Antibody (Catalog # FAB7144A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
  • References:
    1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
    2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
    3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
    4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
    5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
    6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
    7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
  • Long Name:
    B7 Homolog 6
  • Entrez Gene IDs:
    374383 (Human); 102121659 (Cynomolgus Monkey)
  • Alternate Names:
    B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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