Human B7-H6 PE-conjugated Antibody Summary
Accession # Q68D85
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of B7‑H6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7‑H6 PE‑conjugated Monoclonal Antibody (Catalog # FAB7144P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
B7-H6 Specificity is Shown by Flow Cytometry in Knockout Cell Line B7-H6 knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 PE-conjugated Monoclonal Antibody (Catalog # FAB7144P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the B7-H6 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
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- Li, Y. et al. (2011) J. Exp. Med. 208:703.
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- Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Citation for Human B7-H6 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1
Authors: K Walwyn-Bro, K Guldevall, M Saeed, D Pende, B Önfelt, AS MacDonald, DM Davis
J. Immunol., 2018;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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