Human B7-H6 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human B7-H6 in ELISA.
• Source
  Monoclonal Mouse IgG₁ Clone # 875001
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human B7-H6
  Met1-Ser262
  Accession # Q68D85
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• ELISA

  This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB71442).

  This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human B7-H6 DuoSet ELISA (Catalog # DY7144-05) for convenient development of a sandwich ELISA.

• Flow Cytometry
  2.5 µg/10⁶ cells
  See below
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry click image to view larger

Detection of B7‑H6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

ELISA click image to view larger

Human B7‑H6 ELISA Standard Curve. Recombinant Human B7‑H6 protein was serially diluted 2-fold and captured by Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB7144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB71442) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

• Long Name:
  B7 Homolog 6

• Entrez Gene IDs:
  374383 (Human); 102121659 (Cynomolgus Monkey)

• Alternate Names:
  B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1

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