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Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

R&D Systems | Catalog # DBLYS0B

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

6.44 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

The Quantikine Human BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF/BLyS and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human BAFF/BLyS

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 433 1000 2343 474 1118 2111
Standard Deviation 28.2 72.0 78.6 46.8 112 244
CV% 6.5 7.2 3.4 9.9 10.0 11.6

Recovery for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

The recovery of BAFF/BLyS spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 90-103
EDTA Plasma (n=4) 94 84-101
Heparin Plasma (n=4) 94 89-107
Serum (n=4) 93 84-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BAFF/BLyS were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human BAFF/BLyS/TNFSF13B ELISA Linearity

Scientific Data Images for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Human BAFF/BLyS/TNFSF13B ELISA Standard Curve

Human BAFF/BLyS/TNFSF13B ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

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  • Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 01/20/2020
  • Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: A549 human lung carcinoma cell line
    Verified Customer | Posted 05/22/2017

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