Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

(40 citations)
(1 Review)
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    6.44 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human BAFF/BLyS
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC46, Quantikine Immunoassay Control Set 734 for Human BAFF/BLyS - Please Inquire
Product Summary
The Quantikine Human BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF/BLyS and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4581371254549814762854
Standard Deviation17.582.812338.9106256
CV%3.864.87.87.29

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean141462871156482955
Standard Deviation7.5318.837.513.541.796.7
CV%5.34.14.38.78.710.1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean4331000234347411182111
Standard Deviation28.27278.646.8112244
CV%6.57.23.49.91011.6

Recovery

The recovery of BAFF/BLyS spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 90-103
EDTA Plasma (n=4) 94 84-101
Heparin Plasma (n=4) 94 89-107
Serum (n=4) 93 84-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BAFF/BLyS were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BAFF/BLyS/TNFSF13B
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
    • Long Name
      B cell Activating Factor
    • Entrez Gene IDs
      10673 (Human); 24099 (Mouse); 498666 (Rat);
    • Alternate Names
      ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    40 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Comparison of non-invasive biomarkers faecal BAFF, calprotectin and FOBT in discriminating IBS from IBD and evaluation of intestinal inflammation
      Authors: Y Fu, L Wang, C Xie, K Zou, L Tu, W Yan, X Hou
      Sci Rep, 2017;7(1):2669.
      Species: Human
      Sample Type: Faecal
    2. Effect of FCGR polymorphism on the occurrence of late-onset neutropenia and flare-free survival in rheumatic patients treated with rituximab
      Authors: S Ajeganova, D Tesfa, H Hägglund, B Fadeel, I Vedin, AL Zignego, J Palmblad
      Arthritis Res. Ther, 2017;19(1):44.
      Species: Human
      Sample Type: Serum
    3. Intrathecal Th17- and B cell-associated cytokine and chemokine responses in relation to clinical outcome in Lyme neuroborreliosis: a large retrospective study
      Authors: P Gyllemark, P Forsberg, J Ernerudh, AJ Henningsso
      J Neuroinflammation, 2017;14(1):27.
      Species: Human
      Sample Type: Serum
    4. BAFF Index and CXCL13 levels in the cerebrospinal fluid associate respectively with intrathecal IgG synthesis and cortical atrophy in multiple sclerosis at clinical onset
      Authors: M Puthenpara, L Federle, S Miante, A Zito, E Toffanin, S Ruggero, M Ermani, S Pravato, D Poggiali, P Perini, F Rinaldi, P Gallo
      J Neuroinflammation, 2017;14(1):11.
      Species: Human
      Sample Type: Serum
    5. Case-control, exploratory study of cerebrospinal fluid chemokines/cytokines and lymphocyte subsets in childhood Tourette syndrome with positive streptococcal markers
      Authors: MR Pranzatell, ED Tate, TJ Allison
      Cytokine, 2017;96(0):49-53.
      Species: Human
      Sample Type: CSF
    6. Serum B-cell activating factor predicts prognosis in nasal type, extranodal NK/T cell lymphoma
      Cytokine, 2016;88(0):196-198.
      Species: Human
      Sample Type: Serum
    7. A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia
      Haematologica, 2016;101(6):698-706.
      Species: Human
      Sample Type: Serum
    8. Plasmacytoid dendritic cells and myeloid cells differently contribute to BAFF over-expression during primary HIV infection.
      Authors: Borhis G, Burelout C, Chaoul N, Smith N, Goujard C, Meyer L, Paul S, Saoudin H, Hosmalin A, Gilbert C, Herbeuval J, Richard Y
      AIDS, 2016;30(0):365-376.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa.
      Authors: Neill, Daniel R, Saint, Gemma L, Bricio-Moreno, Laura, Fothergill, Joanne L, Southern, Kevin W, Winstanley, Craig, Christmas, Stephen, Slupsky, Joseph R, McNamara, Paul S, Kadioglu, Aras, Flanagan, Brian F
      PLoS ONE, 2014;9(5):e95892.
      Species: Human
      Sample Type: BALF
    10. Neutrophil-mediated IFN activation in the bone marrow alters B cell development in human and murine systemic lupus erythematosus.
      Authors: Palanichamy A, Bauer J, Yalavarthi S, Meednu N, Barnard J, Owen T, Cistrone C, Bird A, Rabinovich A, Nevarez S, Knight J, Dedrick R, Rosenberg A, Wei C, Rangel-Moreno J, Liesveld J, Sanz I, Baechler E, Kaplan M, Anolik J
      J Immunol, 2014;192(3):906-18.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
      Authors: Bornacelly, Adriana, Mercado, Dilia, Acevedo, Nathalie, Caraballo, Luis
      BMC Immunol, 2014;15(0):22.
      Species: Human
      Sample Type: Plasma
    12. B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is related to obesity but not to insulin resistance.
      Authors: Muller N, Schulte D, Hillebrand S, Turk K, Hampe J, Schafmayer C, Brosch M, von Schonfels W, Ahrens M, Zeuner R, Schroder J, Bluher M, Gutschow C, Freitag-Wolf S, Stelmach-Mardas M, Saggau C, Schreiber S, Laudes M
      PLoS ONE, 2014;9(4):e94282.
      Species: Human
      Sample Type: Serum
    13. Serum levels of B-cell activating factor in chronic hepatitis B virus infection: association with clinical diseases.
      Authors: Yang, Cuiling, Li, Na, Wang, Yawen, Zhang, Pingping, Zhu, Qianqian, Li, Fang, Han, Qunying, Lv, Yi, Yu, Ling, Wei, Ping, Liu, Zhengwen
      J Interferon Cytokine Res, 2014;34(10):787-94.
      Species: Human
      Sample Type: Serum
    14. BAFF and BAFF receptor levels correlate with B cell subset activation and redistribution in controlled human malaria infection.
      Authors: Scholzen A, Teirlinck A, Bijker E, Roestenberg M, Hermsen C, Hoffman S, Sauerwein R
      J Immunol, 2014;192(8):3719-29.
      Species: Human
      Sample Type: Plasma
    15. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
      Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
      J Neuroinflammation, 2013;10(0):10.
      Species: Human
      Sample Type: Serum
    16. Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
      Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
      Stem Cells Dev, 2013;22(9):1329-41.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. IFN-beta therapy modulates B-cell and monocyte crosstalk via TLR7 in multiple sclerosis patients.
      Authors: Giacomini E, Severa M, Rizzo F, Mechelli R, Annibali V, Ristori G, Riccieri V, Salvetti M, Coccia E
      Eur J Immunol, 2013;43(7):1963-72.
      Species: Human
      Sample Type: Serum
    18. Role of BAFF in Opsoclonus-Myoclonus syndrome, a bridge between cancer and autoimmunity.
      Authors: Raffaghello L, Fuhlhuber V, Bianchi G, Conte M, Blaes F, Gambini C, Pistoia V
      J Leukoc Biol, 2013;94(1):183-91.
      Species: Human
      Sample Type: Whole Cells
    19. Default in plasma and intestinal IgA responses during acute infection by simian immunodeficiency virus.
      Authors: Chaoul N, Burelout C, Peruchon S, van Buu B, Laurent P, Proust A, Raphael M, Garraud O, Le Grand R, Prevot S, Richard Y
      Retrovirology, 2012;9(0):43.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Plasma
    20. B-cell activating factor levels in rheumatoid arthritis patients in response to treatment with biologics.
      J. Interferon Cytokine Res., 2012;32(7):338-40.
      Species: Human
      Sample Type: Serum
    21. Active chronic sarcoidosis is characterized by increased transitional blood B cells, increased IL-10-producing regulatory B cells and high BAFF levels.
      Authors: Saussine A, Tazi A, Feuillet S, Rybojad M, Juillard C, Bergeron A, Dessirier V, Bouhidel F, Janin A, Bensussan A, Bagot M, Bouaziz J
      PLoS ONE, 2012;7(8):e43588.
      Species: Human
      Sample Type: Serum
    22. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
      Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
      PLoS ONE, 2012;7(7):e40620.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
      Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
      Br. J. Dermatol., 2012;167(2):359-67.
      Species: Human
      Sample Type: Serum
    24. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
      Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
      PLoS ONE, 2011;6(8):e22848.
      Species: Human
      Sample Type: Serum
    25. Significant differences in B-cell subpopulations characterize patients with chronic graft-versus-host disease-associated dysgammaglobulinemia.
      Authors: Kuzmina Z, Greinix HT, Weigl R, Kormoczi U, Rottal A, Frantal S, Eder S, Pickl WF
      Blood, 2011;117(7):2265-74.
      Species: Human
      Sample Type: Plasma
    26. Recovery of B-cell homeostasis after rituximab in chronic graft-versus-host disease.
      Authors: Sarantopoulos S, Stevenson KE, Kim HT
      Blood, 2011;117(7):2275-83.
      Species: Human
      Sample Type: Plasma
    27. B-cell reconstitution and BAFF after alemtuzumab (Campath-1H) treatment of multiple sclerosis.
      Authors: Thompson SA, Jones JL, Cox AL, Compston DA, Coles AJ
      J. Clin. Immunol., 2010;30(1):99-105.
      Species: Human
      Sample Type: Serum
    28. Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection.
      Authors: Sugalski JM, Rodriguez B, Moir S
      J. Immunol., 2010;185(5):3019-27.
      Species: Human
      Sample Type: Serum
    29. Elevated B-cell activating factor BAFF, but not APRIL, correlates with CSF cerebellar autoantibodies in pediatric opsoclonus-myoclonus syndrome.
      Authors: Fuhlhuber V, Bick S, Kirsten A, Hahn A, Gerriets T, Tschernatsch M, Kaps M, Preissner KT, Blaes F, Altenkamper S
      J. Neuroimmunol., 2009;210(1):87-91.
      Species: Human
      Sample Type: Serum
    30. Elevation of B cell-activating factor belonging to the tumour necrosis factor [corrected] family (BAFF) in haemophilia A patients with inhibitor.
      Authors: Takeda T, Sakurai Y, Tatsumi K, Kato J, Kasuda S, Yoshioka A, Shima M
      Thromb. Haemost., 2009;101(2):408-10.
      Species: Human
      Sample Type: Plasma
    31. BAFF and APRIL expression in B-cell chronic lymphocytic leukemia: correlation with biological and clinical features.
      Authors: Bojarska-Junak A, Hus I, Chocholska S, Wasik-Szczepanek E, Sieklucka M, Dmoszynska A, Rolinski J
      Leuk. Res., 2009;33(10):1319-27.
      Species: Human
      Sample Type: Plasma
    32. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
      Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
      Cancer Cell, 2009;16(4):309-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. Rituximab mediates a strong elevation of B-cell-activating factor associated with increased pathogen-specific IgG but not autoantibodies in pemphigus vulgaris.
      Authors: Nagel A, Podstawa E, Eickmann M, Muller HH, Hertl M, Eming R
      J. Invest. Dermatol., 2009;129(9):2202-10.
      Species: Human
      Sample Type: Serum
    34. Altered B-cell homeostasis and excess BAFF in human chronic graft-versus-host disease.
      Authors: Sarantopoulos S, Stevenson KE, Kim HT, Cutler CS, Bhuiya NS, Schowalter M, Ho VT, Alyea EP, Koreth J, Blazar BR, Soiffer RJ, Antin JH, Ritz J
      Blood, 2009;113(16):3865-74.
      Species: Human
      Sample Type: Plasma
    35. BAFF is increased in renal transplant patients following treatment with alemtuzumab.
      Authors: Bloom D, Chang Z, Pauly K
      Am. J. Transplant., 2009;9(8):1835-45.
      Species: Human
      Sample Type: Serum
    36. BAFF may modulate the rate of B-cell repopulation after rituximab therapy for acute renal transplant rejection.
      Authors: Zarkhin V, Li L, Sarwal MM
      Transplantation, 2009;88(10):1229-30.
      Species: Human
      Sample Type: Serum
    37. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps.
      Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP
      J. Allergy Clin. Immunol., 2008;121(6):1385-92, 1392.
      Species: Human
      Sample Type: Nasal Lavage
    38. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
      Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
      Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    39. Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma.
      Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC
      Br. J. Haematol., 2007;139(1):55-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    40. Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment.
      Authors: Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC
      Cancer Res., 2006;66(13):6675-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 40 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 734 for Human BAFF/BLyS

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