Human BAFF R/TNFRSF13C APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C
    Accession # Q96RJ3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BAFF R/TNFRSF13C in Human PBMCs by Flow Cytometry.   Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Goat Anti-Human BAFF R/TNFRSF13C APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1162A) or (B) Normal Goat IgG Allophycocyanin Control (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BAFF R/TNFRSF13C

B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.

  • References:
    1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
    2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
    3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
    4. Thompson, J.S. et al. (2001) Science 14:2108.
  • Long Name:
    B cell Activating Factor Receptor
  • Entrez Gene IDs:
    115650 (Human); 72049 (Mouse)
  • Alternate Names:
    BAFFR; BR3; CD268; TNFRSF13C
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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