Human BAFFR/TNFRSF13C Antibody

Catalog # Availability Size / Price Qty
AF1162
AF1162-SP
Human BAFFR/TNFRSF13C Antibody in Neutralization
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Product Details
Citations (12)
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Human BAFFR/TNFRSF13C Antibody Summary

Species Reactivity
Human
Specificity
Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C
Arg2-Ala71
Accession # Q96RJ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog # 1162-BR)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize BAFF R/TNFRSF13C-mediated inhibition of proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.1 - 1 µg/mL in the presence of 0.3 µg/mL Recombinant Human BAFF R/TNFRSF13C Fc Chimera and 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization View Larger

BAFF R/TNFRSF13C Inhibition of BAFF/BLys/ TNFSF13B-dependent Cell Proliferation and Neutralization by Human BAFF R/TNFRSF13C Antibody. Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog # 1162-BR) inhibits Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) induced proliferation in mouse B cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) activity elicited by Recombinant Human BAFF R/TNFRSF13C Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162). The ND50is typically 0.1-1 µg/mL.

Immunohistochemistry BAFF R/TNFRSF13C in Human Spleen. View Larger

BAFF R/TNFRSF13C in Human Spleen. BAFF R/TNFRSF13C was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow Cytometry Detection of BAFF R/TNFRSF13C in PBMCs by Flow Cytometry. View Larger

Detection of BAFF R/TNFRSF13C in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by APC-conjugated anti-goat secondary antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFFR/TNFRSF13C

B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.

References
  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.
Long Name
B cell Activating Factor Receptor
Entrez Gene IDs
115650 (Human); 72049 (Mouse)
Alternate Names
BAFF R; BAFFR; BR3; CD268; TNFRSF13C

Product Datasheets

Citations for Human BAFFR/TNFRSF13C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. APRIL Binding to BCMA Activates a JNK2-FOXO3-GADD45 Pathway and Induces a G2/M Cell Growth Arrest in Liver Cells.
    Authors: Notas G, Alexaki V, Kampa M, Pelekanou V, Charalampopoulos I, Sabour-Alaoui S, Pediaditakis I, Dessirier V, Gravanis A, Stathopoulos E, Tsapis A, Castanas E
    J Immunol, 2012;189(10):4748-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
    Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Br. J. Dermatol., 2012;167(2):359-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo.
    Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin
    PLoS ONE, 2012;7(10):e47361.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Requirement for BAFF and APRIL during B cell development in GALT.
    Authors: Yeramilli VA, Knight KL
    J. Immunol., 2010;184(10):5527-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
    Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
    Blood, 2008;111(5):2744-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007;178(5):2872-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model.
    Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N
    Clin. Cancer Res., 2007;13(19):5903-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
    Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
    Blood, 2006;107(7):2882-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
    Authors: Pound JD, Batth BK, Johannessen I, Wood K
    J. Immunol., 2005;174(5):3015-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Decreased survival of B cells of HIV-viremic patients mediated by altered expression of receptors of the TNF superfamily.
    Authors: Moir S, Malaspina A, Pickeral OK, Donoghue ET, Vasquez J, Miller NJ, Krishnan SR, Planta MA, Turney JF, Justement JS, Kottilil S, Dybul M, Mican JM, Kovacs C, Chun TW, Birse CE, Fauci AS
    J. Exp. Med., 2004;200(7):587-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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