Human beta-TrCP1/BTRC Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # FAB7675AFP405

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 763524
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Product Specifications

Specificity

Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human beta-TrCP1/BTRC Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: beta-TrCP1/BTRC

beta -TrCP1, also called BTRC ( beta -transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta -TrCP1 can form homodimers and heterodimers with beta -TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta -TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta -TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta -TrCP1, respectively.

Long Name

Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

Alternate Names

betaTrCP1, BTRC, BTRCP, E3RSIkappaB, FBW1A, FBXW1A, FWD1

Entrez Gene IDs

8945 (Human); 12234 (Mouse); 361765 (Rat)

Gene Symbol

BTRC

UniProt

Additional beta-TrCP1/BTRC Products

Product Documents for Human beta-TrCP1/BTRC Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human beta-TrCP1/BTRC Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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